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Laboratory of Biochemistry and Functional Proteomics

Recent Papers (2014-2008)

 

Meneghini C, Leboffe L, Bionducci M, Fanali G, Meli M, Colombo G, Fasano M, Ascenzi P, Mobilio S. The Five-To-Six-Coordination Transition of Ferric Human Serum Heme-Albumin Is Allosterically-Modulated by Ibuprofen and Warfarin: A Combined XAS and MD Study. PLoS One. 2014 Aug 25;9(8):e104231. doi:10.1371/journal.pone.0104231. eCollection 2014. PubMed PMID: 25153171; PubMed Central PMCID: PMC4143227.

Di Muzio E, Polticelli F, Trezza V, Fanali G, Fasano M, Ascenzi P. Imatinib binding to human serum albumin modulates heme association and reactivity. Arch Biochem Biophys. 2014 Oct 15;560:100-12. doi: 10.1016/j.abb.2014.07.001. PubMed PMID: 25057771.

Alberio T, Mammucari C, D'Agostino G, Rizzuto R, Fasano M. Altered dopamine homeostasis differentially affects mitochondrial voltage-dependent anion channels turnover. Biochim Biophys Acta. 2014 Sep;1842(9):1816-22. doi: 10.1016/j.bbadis.2014.06.033. Epub 2014 Jul 3. PubMed PMID: 24998333.

Alberio T, McMahon K, Cuccurullo M, Gethings LA, Lawless C, Zibetti M, Lopiano L, Vissers JP, Fasano M. Verification of a Parkinson's disease protein signature in T-lymphocytes by multiple reaction monitoring. J Proteome Res. 2014 Aug 1;13(8):3554-61. doi: 10.1021/pr401142p. Epub 2014 Jul 1. PubMed PMID: 24946097.

Roncada P, Modesti A, Timperio AM, Bini L, Castagnola M, Fasano M, Urbani A. One medicine--one health--one biology and many proteins: proteomics on the verge of the One Health approach. Mol Biosyst. 2014 Jun;10(6):1226-7. doi: 10.1039/c4mb90011a. Epub 2014 Apr 29. PubMed PMID: 24777557.

Alberio T, Bondi H, Colombo F, Alloggio I, Pieroni L, Urbani A, Fasano M. Mitochondrial proteomics investigation of a cellular model of impaired dopamine homeostasis, an early step in Parkinson's disease pathogenesis. Mol Biosyst. 2014 Jun;10(6):1332-44. doi: 10.1039/c3mb70611g. Epub 2014 Mar 28. PubMed PMID: 24675778.

Ascenzi P, Tundo GR, Fanali G, Coletta M, Fasano M. Warfarin modulates the nitrite reductase activity of ferrous human serum heme-albumin. J Biol Inorg Chem. 2013 Dec;18(8):939-46. doi: 10.1007/s00775-013-1040-2. Epub 2013 Sep 15. PubMed PMID: 24037275.

Ascenzi P, Coletta A, Cao Y, Trezza V, Leboffe L, Fanali G, Fasano M, Pesce A, Ciaccio C, Marini S, Coletta M. Isoniazid inhibits the heme-based reactivity of Mycobacterium tuberculosis truncated hemoglobin N. PLoS One. 2013 Aug 1;8(8):e69762. doi: 10.1371/journal.pone.0069762. Print 2013. PubMed PMID: 23936350; PubMed Central PMCID: PMC3731299.

Urbani A, De Canio M, Palmieri F, Sechi S, Bini L, Castagnola M, Fasano M, Modesti A, Roncada P, Timperio AM, Bonizzi L, Brunori M, Cutruzzolà F, De Pinto V, Di Ilio C, Federici G, Folli F, Foti S, Gelfi C, Lauro D, Lucacchini A, Magni F, Messana I, Pandolfi PP, Papa S, Pucci P, Sacchetta P; Italian Mt-Hpp Study Group-Italian Proteomics Association (www.itpa.it). The mitochondrial Italian Human Proteome Project initiative (mt-HPP). Mol Biosyst. 2013 Aug;9(8):1984-92. doi: 10.1039/c3mb70065h. Epub 2013 May 28. PubMed PMID: 23712443.

Urbani A, Castagnola M, Fasano M, Bini L, Modesti A, Timperio AM, Roncada P. Digital and analogical reality in proteomics investigation. Mol Biosyst. 2013 Jun;9(6):1062-3. doi: 10.1039/c3mb90013d. Epub 2013 Apr 30. PubMed PMID: 23629630.

Ascenzi P, di Masi A, Leboffe L, Alberio T, Fanali G, Fasano M. Molecular phylogenetic analyses of albuminoids reveal the molecular evolution of allosteric properties. IUBMB Life. 2013 Jun;65(6):544-9. doi: 10.1002/iub.1164. Epub 2013 Apr 9. PubMed PMID: 23568641.

Bocedi A, De Sanctis G, Ciaccio C, Tundo GR, Di Masi A, Fanali G, Nicoletti FP, Fasano M, Smulevich G, Ascenzi P, Coletta M. Reciprocal allosteric modulation of carbon monoxide and warfarin binding to ferrous human serum heme-albumin. PLoS One. 2013;8(3):e58842. doi: 10.1371/journal.pone.0058842. Epub 2013 Mar 21. PubMed PMID: 23555601; PubMed Central PMCID: PMC3605432.

Falvella FS, Alberio T, Noci S, Santambrogio L, Nosotti M, Incarbone M, Pastorino U, Fasano M, Dragani TA. Multiple isoforms and differential allelic expression of CHRNA5 in lung tissue and lung adenocarcinoma. Carcinogenesis. 2013 Jun;34(6):1281-5. doi: 10.1093/carcin/bgt062. Epub 2013 Feb 19. PubMed PMID: 23430818.

Alberio T, Bucci EM, Natale M, Bonino D, Di Giovanni M, Bottacchi E, Fasano M. Parkinson's disease plasma biomarkers: an automated literature analysis followed by experimental validation. J Proteomics. 2013 Sep 2;90:107-14. doi: 10.1016/j.jprot.2013.01.025. Epub 2013 Feb 4. PubMed PMID: 23385359.

Fanali G, Fasano M, Ascenzi P, Zingg JM, Azzi A. α-Tocopherol binding to human serum albumin. Biofactors. 2013.

http://www.ncbi.nlm.nih.gov/pubmed/23355326.

 

Alberio T, Pippione AC, Zibetti M, Olgiati S, Cecconi D, Comi C, Lopiano L, Fasano M. Discovery and verification of panels of T-lymphocyte proteins as biomarkers of Parkinson's disease. Sci Rep. 2012; 2:953. doi:10.1038/srep00953.

http://www.ncbi.nlm.nih.gov/pubmed/23233872.

 

Polati R, Castagna A, Bossi AM, Alberio T, De Domenico I, Kaplan J, Timperio AM, Zolla L, Gevi F, D'Alessandro A, Brunch R, Olivieri O, Girelli D. Murine macrophages response to iron. J Proteomics. 2012; 76 Spec No.:10-27.

http://www.ncbi.nlm.nih.gov/pubmed/22835775.

 

Alberio T, Pippione AC, Comi C, Olgiati S, Cecconi D, Zibetti M, Lopiano L, Fasano M. Dopaminergic therapies modulate the T-CELL proteome of patients with Parkinson's disease. IUBMB Life. 2012; 64:846-52.

http://www.ncbi.nlm.nih.gov/pubmed/22815142.

 

Fanali G, Cao Y, Ascenzi P, Fasano M. Mn(II) binding to human serum albumin: A (1)H-NMR relaxometric study. J Inorg Biochem. 2012; 117:198-203.

http://www.ncbi.nlm.nih.gov/pubmed/23099538.

 

Ascenzi P, Fasano M. Pseudo-enzymatic hydrolysis of 4-nitrophenyl myristate by human serum albumin. Biochem Biophys Res Commun. 2012; 422:219-23.

http://www.ncbi.nlm.nih.gov/pubmed/22560903.

 

Ascenzi P, Gioia M, Fanali G, Coletta M, Fasano M. Pseudo-enzymatic hydrolysis of 4-nitrophenyl acetate by human serum albumin: pH-dependence of rates of individual steps. Biochem Biophys Res Commun. 2012; 424:451-5.

http://www.ncbi.nlm.nih.gov/pubmed/22771811.

 

Fanali G, Ascenzi P, Bernardi G, Fasano M. Sequence analysis of serum albumins reveals the molecular evolution of ligand recognition properties. J Biomol Struct Dyn. 2012; 29:691-701.

http://www.ncbi.nlm.nih.gov/pubmed/22545999.

 

Urbani A, Modesti A, Timperio AM, Bini L, Castagnola M, Fasano M, Roncada P. Integrative proteomics: perspective in complex system interpretation. Mol Biosyst. 2012; 8:951-2.

http://www.ncbi.nlm.nih.gov/pubmed/22377959.

 

Alberio T, Lopiano L, Fasano M. Cellular models to investigate biochemical pathways in Parkinson's disease. FEBS J. 2012; 279:1146-55.

http://www.ncbi.nlm.nih.gov/pubmed/22314200.

 

Fasano M. Biochemistry of Parkinson's disease--insights from cellular models, animal models and human tissue specimens obtained by autopsy. FEBS J. 2012; 279:1145.

http://www.ncbi.nlm.nih.gov/pubmed/22269014.

 

Fanali G, di Masi A, Trezza V, Marino M, Fasano M, Ascenzi P. Human serum albumin: from bench to bedside. Mol Aspects Med. 2012; 33:209-90.

http://www.ncbi.nlm.nih.gov/pubmed/22230555.

 

Alberio T, Fasano M. Proteomics in Parkinson's disease: An unbiased approach towards peripheral biomarkers and new therapies. J Biotechnol. 2011; 156:325-37. 

http://www.ncbi.nlm.nih.gov/pubmed/21925549.

 

Cao Y, Nicoletti FP, De Sanctis G, Bocedi A, Ciaccio C, Gullotta F, Fanali G, Tundo GR, di Masi A, Fasano M, Smulevich G, Ascenzi P, Coletta M. Evidence for pH-dependent multiple conformers in iron(II) heme-human serum albumin: spectroscopic and kinetic investigation of carbon monoxide binding. J Biol Inorg Chem. 2012; 17:133-47.

http://www.ncbi.nlm.nih.gov/pubmed/21894504.

 

Ascenzi P, Cao Y, Tundo GR, Coletta M, Fanali G, Fasano M. Ibuprofen and warfarin modulate allosterically ferrous human serum heme-albumin nitrosylation. Biochem Biophys Res Commun. 2011; 411:185-9.

http://www.ncbi.nlm.nih.gov/pubmed/21726535.

 

Fanali G, Cao Y, Ascenzi P, Trezza V, Rubino T, Parolaro D, Fasano M. Binding of δ9-tetrahydrocannabinol and diazepam to human serum albumin. IUBMB Life. 2011; 63:446-51.

http://www.ncbi.nlm.nih.gov/pubmed/21557446.

 

Alberio T, Anchieri C, Piacentini L, Gentile G, Simmaco M, Biasin M, Fasano M. Proteomic characterization of Jurkat T leukemic cells after dopamine stimulation: A model of circulating dopamine-sensitive cells. Biochimie. 2011; 93:892-8.

http://www.ncbi.nlm.nih.gov/pubmed/21300132.

 

Urbani A, Modesti A, Timperio AM, Bini L, Castagnola M, Fasano M, Roncada P. Proteomics and renaissance: accounts of the V Italian Proteomics Association Congress, Florence 2010. Mol Biosyst. 2011; 7:577-8.

http://www.ncbi.nlm.nih.gov/pubmed/21301747.

 

Ascenzi P, Gullotta F, Gioia M, Coletta M, Fasano M. O(2)-mediated oxidation of ferrous nitrosylated human serum heme-albumin is limited by nitrogen monoxide  dissociation. Biochem Biophys Res Commun. 2011

http://www.ncbi.nlm.nih.gov/pubmed/21296054.

 

di Masi A, Gullotta F, Bolli A, Fanali G, Fasano M, Ascenzi P. Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme-albumin. FEBS J. 2011; 278:654-62.

http://www.ncbi.nlm.nih.gov/pubmed/21205199.

 

Ascenzi P, Bolli A, Gullotta F, Fanali G, Fasano M. Drug binding to Sudlow's site I impairs allosterically human serum heme-albumin-catalyzed peroxynitrite detoxification. IUBMB Life. 2010; 62:776-80.

http://www.ncbi.nlm.nih.gov/pubmed/20979209.

 

Alberio T, Bossi AM, Milli A, Parma E, Gariboldi MB, Tosi G, Lopiano L, Fasano M. Proteomic analysis of dopamine and α-synuclein interplay in a cellular model of Parkinson's disease pathogenesis. FEBS J. 2010; 277:4909-19.

http://www.ncbi.nlm.nih.gov/pubmed/20977677.

 

Ascenzi P, Bolli A, di Masi A, Tundo GR, Fanali G, Coletta M, Fasano M. Isoniazid and rifampicin inhibit allosterically heme binding to albumin and peroxynitrite isomerization by heme-albumin. J Biol Inorg Chem. 2010.

http://www.ncbi.nlm.nih.gov/pubmed/20865291.

 

Alberio T, Colapinto M, Natale M, Ravizza R, Gariboldi MB, Bucci EM, Lopiano L, Fasano M. Changes in the two-dimensional electrophoresis pattern of the Parkinson's disease related protein DJ-1 in human SH-SY5Y neuroblastoma cells after dopamine treatment. IUBMB Life. 2010.

http://www.ncbi.nlm.nih.gov/pubmed/20836127.

 

Bolli A, Marino M, Rimbach G, Fanali G, Fasano M, Ascenzi P. Flavonoid binding to human serum albumin. Biochem Biophys Res Commun. 2010; 398: 444-449.

http://www.ncbi.nlm.nih.gov/pubmed/20599706.

 

Ascenzi P, Cao Y, di Masi A, Gullotta F, De Sanctis G, Fanali G, Fasano M, Coletta M. Reductive nitrosylation of ferric human serum heme-albumin. FEBS J. 2010; 277:2474-2485.

http://www.ncbi.nlm.nih.gov/pubmed/20456498.

 

Fanali G, Rampoldi V, di Masi A, Bolli A, Lopiano L, Ascenzi P, Fasano M. Binding of anti-Parkinson's disease drugs to human serum albumin is allosterically modulated. IUBMB Life. 2010; 62: 371-376.

http://www.ncbi.nlm.nih.gov/pubmed/20225277.

 

Natale M, Bonino D, Consoli P, Alberio T, Ravid RG, Fasano M, Bucci EM. A meta-analysis of two-dimensional electrophoresis pattern of the Parkinson's disease-related protein DJ-1. Bioinformatics. 2010; 26: 946-952.

http://www.ncbi.nlm.nih.gov/pubmed/20172943.

 

Ascenzi P, Fasano M. Allostery in a monomeric protein: The case of human serum albumin. Biophys Chem. 2010; 148: 16-22.

http://www.ncbi.nlm.nih.gov/pubmed/20346571.

 

Ascenzi P, Fasano M. Serum heme-albumin: an allosteric protein. IUBMB Life. 2009; 61: 1118-1122.

http://www.ncbi.nlm.nih.gov/pubmed/19946891.

 

Ascenzi P, di Masi A, Coletta M, Ciaccio C, Fanali G, Nicoletti FP, Smulevich G, Fasano M. Ibuprofen impairs allosterically peroxynitrite isomerization by ferric human serum heme-albumin. J Biol Chem. 2009; 284: 31006-31017.

http://www.ncbi.nlm.nih.gov/pubmed/19734142.

 

Ascenzi P, di Masi A, De Sanctis G, Coletta M, Fasano M. Ibuprofen modulates allosterically NO dissociation from ferrous nitrosylated human serum heme-albumin by binding to three sites. Biochem Biophys Res Commun. 2009; 387: 83-6.

http://www.ncbi.nlm.nih.gov/pubmed/19559669.

 

Baroni S, Pariani G, Fanali G, Longo D, Ascenzi P, Aime S, Fasano M. Thermodynamic analysis of hydration in human serum heme-albumin. Biochem Biophys  Res Commun. 2009; 385: 385-9.

http://www.ncbi.nlm.nih.gov/pubmed/19464261.

 

Rubino T, Realini N, Braida D, Alberio T, Capurro V, Viganò D, Guidali C, Sala M, Fasano M, Parolaro D. The depressive phenotype induced in adult female rats by adolescent exposure to THC is associated with cognitive impairment and altered neuroplasticity in the prefrontal cortex. Neurotox Res. 2009; 15: 291-302.

http://www.ncbi.nlm.nih.gov/pubmed/19384563.

 

Fanali G, Pariani G, Ascenzi P, Fasano M. Allosteric and binding properties of Asp1-Glu382 truncated recombinant human serum albumin--an optical and NMR spectroscopic investigation. FEBS J. 2009; 276:2241-2250.

http://www.ncbi.nlm.nih.gov/pubmed/19298387.

 

Willems P, Magri V, Cretnik M, Fasano M, Jakubowska A, Levanat S, Lubinski J, Marras E, Musani V, Thierens H, Vandersickel V, Perletti G, Vral A. Characterization of the c.190T>C missense mutation in BRCA1 codon 64 (Cys64Arg). Int J Oncol. 2009; 34: 1005-1015.

http://www.ncbi.nlm.nih.gov/pubmed/19287957.

 

Mila S, Giuliano Albo A, Corpillo D, Giraudo S, Zibetti M, Bucci EM, Lopiano L, Fasano M. Lymphocyte proteomics of Parkinson's disease patients reveals cytoskeletal protein dysregulation and oxidative stress. Biomarkers Med. 2009; 3: 117-128.

http://www.ncbi.nlm.nih.gov/pubmed/20477505.

 

Fanali G, De Sanctis G, Gioia M, Coletta M, Ascenzi P, Fasano M. Reversible two-step unfolding of heme-human serum albumin: a (1)H-NMR relaxometric and circular dichroism study. J Biol Inorg Chem. 2009; 14: 209-217.

http://www.ncbi.nlm.nih.gov/pubmed/18936983.

 

Fasano M, Alberio T, Lopiano L. Peripheral biomarkers of Parkinson's disease as early reporters of central neurodegeneration. Biomarkers Med. 2008; 2: 465-78.

http://www.ncbi.nlm.nih.gov/pubmed/20477424.

 

Nicoletti FP, Howes BD, Fittipaldi M, Fanali G, Fasano M, Ascenzi P, Smulevich G. Ibuprofen induces an allosteric conformational transition in the heme complex of human serum albumin with significant effects on heme ligation. J Am Chem Soc. 2008; 130: 11677-88.

http://www.ncbi.nlm.nih.gov/pubmed/18681435.

 

Ascenzi P, Imperi F, Coletta M, Fasano M. Abacavir and warfarin modulate allosterically kinetics of NO dissociation from ferrous nitrosylated human serum heme-albumin. Biochem Biophys Res Commun. 2008; 369: 686-91.

http://www.ncbi.nlm.nih.gov/pubmed/18307975.

 

Fasano M, Lopiano L. Alpha-synuclein and Parkinson's disease: a proteomic view. Expert Rev Proteomics. 2008; 5: 239-48.

http://www.ncbi.nlm.nih.gov/pubmed/18466054.

 

Fasano M, Alberio T, Colapinto M, Mila S, Lopiano L. Proteomics as a tool to  investigate cell models for dopamine toxicity. Parkinsonism Relat Disord. 2008; 14 Suppl 2: S135-8.

http://www.ncbi.nlm.nih.gov/pubmed/18585082.